Monitoring the initiation and kinetics of human dendritic cell-induced polarization of autologous naive CD4+ T cells.

Monitoring the initiation and kinetics of human dendritic cell-induced polarization of autologous naive CD4+ T cells.

Blog Article

A crucial step in generating de novo immune responses is the polarization of naive cognate CD4+ T cells by pathogen-triggered dendritic cells (DC).In the human setting, standardized DC-dependent systems are lacking to study molecular events during the initiation of a naive CD4+ T cell response.We developed a TCR-restricted assay to compare different pathogen-triggered human DC for their capacities to instruct functional differentiation of autologous, naive CD4+ T cells.We demonstrated that this methodology can be applied to compare Lower Control Panel differently matured DC in terms of kinetics, direction, and magnitude of the naive CD4+ T cell response.

Furthermore, we showed the applicability of this assay to study the T cell polarizing capacity of low-frequency blood-derived DC populations Lip Gloss directly isolated ex vivo.This methodology for addressing APC-dependent instruction of naive CD4+ T cells in a human autologous setting will provide researchers with a valuable tool to gain more insight into molecular mechanisms occurring in the early phase of T cell polarization.In addition, it may also allow the study of pharmacological agents on DC-dependent T cell polarization in the human system.